![]() The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. ![]() For this purpose, the broth (with added agar) should be used soon after sterilisation, or, heated and cooled just before inoculation. The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium spp. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner. Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeias 1,4,5,6. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc. Sterilise by autoclaving at 121☌ for 15 minutes.Ī highly nutritious versatile medium which is recommended for general laboratory use. Our approach to manufacturing ensures all sterility media and rinse fluids provide the highest level of clarity for improved reading accuracy, thus significantly reducing the risk of incorrect interpretation and false results.TRYPTONE SOYA BROTH (Casein soya bean digest medium) EP/USP/JP/BPĪ highly nutritious general purpose medium for the growth of bacteria and fungiĪdd 30g to 1 litre of water (purified as required), mix well and distribute into final containers. Each lot is certified for pH, sterility and growth promotion using ATCC® strains specified by the USP. Hardy Diagnostics' sterility media and rinse solutions are manufactured in an ISO 13485 environmentally controlled production center. The product may be used and tested up to the expiration date on the product label and incubated for the recommended quality control incubation times. The expiration dating on the product label applies to the product in its intact packaging when stored as directed. Protect from light, excessive heat, moisture, and freezing. Media should not be used if there are any signs of deterioration (discoloration), contamination, or if the expiration date has passed. Formula Ingredients per liter of deionized water: The dipotassium phosphate is included in the formulation as a buffer to maintain the pH. Dextrose is incorporated as an energy source. Sodium chloride is added to maintain the osmotic equilibrium. This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. ![]() Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources supporting the growth of the majority of bacteria and fungi. ![]() This product is not intended to be used for the diagnosis of human disease. Pharmacopeia National Formulary (USP) describes its use for sterility testing. Hardy Diagnostics Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of bacteria and fungi. Also known as TSB or Soybean-Casein Digest Broth, USP. ![]()
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